Left: Conventional Confocal Right: STELLARIS platform. Excitation up to 790 nm with WLL Detection up to 850 nm Mitotic COS7 Cells SiR-Actin (ex: 647 nm, em: 657-740 nm) AF750-Tom20 (ex: 750 nm, em: 760-790 nm) AF790-memb (ex: 790 nm, em: 810-850 nm) Sample Courtesy: Jana Döhner, Urs Ziegler, University of Zürich
Power – See more
Leica Stellaris – Get Closer to the Truth – Confocal ReImagined
The synergy between the new generation of Power HyD detectors, the completely optimized beam path, and the unique White Light Laser gives you perfected imaging performance. Your answers are clearer, derived from brighter signals, more contrast and astounding detail from even multiple low abundance labels. Our new Power HyD detectors provide you with:
- Enhanced image quality: STELLARIS provides an ideal combination of brightness, resolution and contrast.
- Complete spectral freedom: Our next-generation White Light Lasers which allow a perfect match to the fluorochrome allows you to simultaneously use up to 8 single excitation lines from 440 nm to 790 nm.
- Gentle Live Cell Imaging: Preserve your sample’s integrity through efficient signal acquisition at the lowest required levels of illumination
Key technical advancements:
Power HyD detectors deliver a Photon Detection Efficiency (PDE) of up to 56% with the most commonly used fluorophores. At least 2x more efficiency than what can be achieved with conventional alkali photomultipliers. PDE in the extended red range is even 3x higher.- Near Infrared (NIR) detection range is extended to 850 nm, accommodating 3 additional detection colors
- Up to 67% improved dynamic range compared to current state-of-the-art detectors* (*Comparison of max counts SP8 HyD vs Stellaris HyD X and HyD R in photon counting mode (CW))
- Up to 8 excitation lines from 440 nm to 790 nm can be used simultaneously with the next generation White Light Lasers
- A redesigned beam path that maximises transmission efficiency
Potential – Discover more
STELLARIS includes Leica’s new and unique TauSense technology allowing you to extract a new dimension of information from every sample and increase the scientific impact of your research using lifetime-based data to explore the function of molecules in their cellular context. Our new TauSense technology gives you access to:
- New Dimension of Imaging: TauContrast gives you immediate access to functional information such as metabolic status, pH and ion concentration
- Improve Image Quality: Maximize detection efficiency by removing unwanted autofluorescence while preserving the desired signal with TauGating
- Multiplex Beyond Spectral Options: Separate species with TauSeparation even when their emissions fully overlap
Key technical advancements:
- Pixel by pixel readout of the mean photon arrival time, simultaneous with intensity detection
- Up to 16 time-gates simultaneously, digitally tunable
- Life-time based component separation algorithm
Productivity – Do more
STELLARIS uses its new ImageCompass new smart user interface to provide users with an easier and intuitive way to set up even the most complex experiments. Additionally, your confocal experiments are optimized with our LIGHTNING Super Resolution technology and our NAVIGATOR sample overview.
- Simply Fast: Superb image quality in real-time and at full speed by combining Super Resolution LIGHTNING, our resonant scanner, and our new rolling average
- Intuitive User Interface: ImageCompass guides you from your experiment setup through acquisition
- Streamlined Experiment Set Up: Easily be in full control of your experimental setup with a few clicks
Key technical advancements:
- Set up a multicolor experiment with 1 click per fluorophore
- Maximal signal yield through optimal acquisition settings, automatically selected
- Uncompromised temporal resolution up to 420 frames/sec maintaining optimal image quality, further enhanced by LIGHTNING
- Access to fluorescence lifetime information in 1 click
