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Oulu – Technical Service

Aapistie 7 B
90220 Oulu

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Contact us Technical support and service
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New tools for CAR-T therapy development – Read more!

Hot Products from ACROBiosystems!

CAR-specific Monoclonal Antibody to Detect Anti-CD20 CAR Expression

  • Specifically recognize the antigen-binding site of Anti-CD20 (Rituximab) CAR with high affinity
  • Flow Cytometry Validated
  • Protocols are offered for FREE

ACROBiosystems Target Antigens for Evaluation of CAR Expression

ACROBiosystems has developed an extensive collection of recombinant proteins to support CAR-T therapy development. Available also difficult-to-express proteins such as BCMA, CD19, ROR1 and EGFRVIII.

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Measure CAR T-Mediated Cytotoxicity using Tracer and Viability Dyes with Celigo!

 

Celigo is a micro-well plate based cell counting instrument  with bright field plus 2-4 fluorescent channels for simple cell-base assays with flow-like data output.

 

Suitable for different applications in Immuno-Oncology, 3D Tumor Spheroid Modelling, Stem Cell Research, Cell Line Development and Biologics Production, and Virology Assays.

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CAR-T target and effector cell fluorescent and bright field overlay images with Celigo
Tumor cells were labeled with CFSE prior to mixing with CAR-T cells. Yellow cells in the red/green merge images represent dead target tumor cells. Propidium iodide only positive cells are dead CAR-T cells. The live target tumor cells are green in color.


Fluorescent intensity gating for cytotoxicity percentage with Celigo
The number of dead target cells increased as the E:T ratio increased, which can be visually seen in the scatter plot. The gating allowed the measurement of the number of live and dead target cells to calculate the final cytotoxicity percentage.

Read more about using Celigo with CAR-T!

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